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A slimy non-spore-forming bacterium strain SAF-C isolated from bean stem and root was motile with flagella and identified to one of Agrobacterium radiobacter. Studies were made on the conditions necessary for maximal production of this acidic succinoglucan polysaccharide by this strain in shaken culture. Much production was observed with yeast extract, (NH₄)₂HPO₄, distillers´ dried solubles(D.D.S.), as nitrogen source in the medium composed of 4% glucose, 0.1% nitrogen source, 0.3% CaCO₃. The yield was greatest with yeast extract and decreased in order with the above nitrogen source from 22.9% to 9.6 percent. A polysaccharide was produced in a yield of about 25% in a medium composed of 3% glucose, 0.4% D.D.S., 0.5% K₂HPO₄, 0.01% MgSO₄·7H₂O.
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